Chlorella sp. modulates the glutathione mediated detoxification and S-adenosylmethionine dependent methyltransferase to counter arsenic toxicity in Oryza sativa L.
Identifieur interne : 000087 ( Main/Exploration ); précédent : 000086; suivant : 000088Chlorella sp. modulates the glutathione mediated detoxification and S-adenosylmethionine dependent methyltransferase to counter arsenic toxicity in Oryza sativa L.
Auteurs : Ruma Ranjan [Inde] ; Navin Kumar [Inde] ; Ambedkar Gautam [Inde] ; Arvind Kumar Dubey [Inde] ; Shyam Narayan Pandey [Inde] ; Shekhar Mallick [Inde]Source :
- Ecotoxicology and environmental safety [ 1090-2414 ] ; 2020.
Abstract
The present study investigates the role of Chlorella sp. in the mitigation of arsenic (iAs) induced toxicity in Oryza sativa L. The study shows, co-culture of rice seedlings with Chlorella sp. reduced the iAs accumulation, simultaneously improving the growth of seedlings under iAs treatments. While treatment with As(III) and As(V) (60 µM) alone, inflicted toxicity in rice seedlings, manifested as significant enhancement in stress markers levels (TBRAS and H2O2), this coincided with the shifting of cellular reduced state to oxidized state (reduced GSH/GSSG ratio). Contrarily, co-culturing rice seedlings with Chlorella sp. under iAs toxicity, reduced these stress markers and recovered the GSH/GSSG ratio. The GSH dependent antioxidant enzymes i.e. GR and GPX activities also exhibited significant enhancement upon co-culturing rice seedlings with Chlorella sp. against iAs stress. Simultaneously, the expression of four thiol dependent GRX genes, i.e. GRX13950, GRX35340, GRX12190 and GRX07950 were enhanced against As(III) and As(V) (60 µM), which reduced upon co-culturing with Chlorella sp. A similar trend was also observed with the expression of GST genes, where the co-culture with Chlorella sp. significantly reduced the genes expression of two isoforms (GST 38600 and GST 38610). On the contrary, the expression of S-adenosylmethionine dependent methyltransferases (SAMT) gene in rice seedlings was enhanced upon co-culturing with the Chlorella sp. against iAs stress. Overall, the results demonstrate that the rice seedlings when co-culture with Chlorella sp. ameliorates iAs toxicity through GSH dependent detoxification pathway, evident from the enhanced expression of GRX, GST, SAMT genes and activity of GSH dependent antioxidant enzymes (GR and GPX) in the rice seedlings.
DOI: 10.1016/j.ecoenv.2020.111418
PubMed: 33045435
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Electronic address: shekharm@nbri.res.in.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>CSIR-National Botanical Research Institute, Lucknow</wicri:regionArea><wicri:noRegion>Lucknow</wicri:noRegion></affiliation></author></analytic><series><title level="j">Ecotoxicology and environmental safety</title><idno type="eISSN">1090-2414</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The present study investigates the role of Chlorella sp. in the mitigation of arsenic (iAs) induced toxicity in Oryza sativa L. The study shows, co-culture of rice seedlings with Chlorella sp. reduced the iAs accumulation, simultaneously improving the growth of seedlings under iAs treatments. While treatment with As<sup>(III)</sup> and As<sup>(V)</sup> (60 µM) alone, inflicted toxicity in rice seedlings, manifested as significant enhancement in stress markers levels (TBRAS and H<sub>2</sub>O<sub>2</sub>), this coincided with the shifting of cellular reduced state to oxidized state (reduced GSH/GSSG ratio). Contrarily, co-culturing rice seedlings with Chlorella sp. under iAs toxicity, reduced these stress markers and recovered the GSH/GSSG ratio. The GSH dependent antioxidant enzymes i.e. GR and GPX activities also exhibited significant enhancement upon co-culturing rice seedlings with Chlorella sp. against iAs stress. Simultaneously, the expression of four thiol dependent GRX genes, i.e. GRX13950, GRX35340, GRX12190 and GRX07950 were enhanced against As<sup>(III)</sup> and As<sup>(V)</sup> (60 µM), which reduced upon co-culturing with Chlorella sp. A similar trend was also observed with the expression of GST genes, where the co-culture with Chlorella sp. significantly reduced the genes expression of two isoforms (GST 38600 and GST 38610). On the contrary, the expression of S-adenosylmethionine dependent methyltransferases (SAMT) gene in rice seedlings was enhanced upon co-culturing with the Chlorella sp. against iAs stress. Overall, the results demonstrate that the rice seedlings when co-culture with Chlorella sp. ameliorates iAs toxicity through GSH dependent detoxification pathway, evident from the enhanced expression of GRX, GST, SAMT genes and activity of GSH dependent antioxidant enzymes (GR and GPX) in the rice seedlings.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">33045435</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1090-2414</ISSN><JournalIssue CitedMedium="Internet"><Volume>208</Volume><PubDate><Year>2020</Year><Month>Oct</Month><Day>09</Day></PubDate></JournalIssue><Title>Ecotoxicology and environmental safety</Title><ISOAbbreviation>Ecotoxicol Environ Saf</ISOAbbreviation></Journal><ArticleTitle>Chlorella sp. modulates the glutathione mediated detoxification and S-adenosylmethionine dependent methyltransferase to counter arsenic toxicity in Oryza sativa L.</ArticleTitle><Pagination><MedlinePgn>111418</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0147-6513(20)31255-0</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ecoenv.2020.111418</ELocationID><Abstract><AbstractText>The present study investigates the role of Chlorella sp. in the mitigation of arsenic (iAs) induced toxicity in Oryza sativa L. The study shows, co-culture of rice seedlings with Chlorella sp. reduced the iAs accumulation, simultaneously improving the growth of seedlings under iAs treatments. While treatment with As<sup>(III)</sup> and As<sup>(V)</sup> (60 µM) alone, inflicted toxicity in rice seedlings, manifested as significant enhancement in stress markers levels (TBRAS and H<sub>2</sub>O<sub>2</sub>), this coincided with the shifting of cellular reduced state to oxidized state (reduced GSH/GSSG ratio). Contrarily, co-culturing rice seedlings with Chlorella sp. under iAs toxicity, reduced these stress markers and recovered the GSH/GSSG ratio. The GSH dependent antioxidant enzymes i.e. GR and GPX activities also exhibited significant enhancement upon co-culturing rice seedlings with Chlorella sp. against iAs stress. Simultaneously, the expression of four thiol dependent GRX genes, i.e. GRX13950, GRX35340, GRX12190 and GRX07950 were enhanced against As<sup>(III)</sup> and As<sup>(V)</sup> (60 µM), which reduced upon co-culturing with Chlorella sp. A similar trend was also observed with the expression of GST genes, where the co-culture with Chlorella sp. significantly reduced the genes expression of two isoforms (GST 38600 and GST 38610). On the contrary, the expression of S-adenosylmethionine dependent methyltransferases (SAMT) gene in rice seedlings was enhanced upon co-culturing with the Chlorella sp. against iAs stress. Overall, the results demonstrate that the rice seedlings when co-culture with Chlorella sp. ameliorates iAs toxicity through GSH dependent detoxification pathway, evident from the enhanced expression of GRX, GST, SAMT genes and activity of GSH dependent antioxidant enzymes (GR and GPX) in the rice seedlings.</AbstractText><CopyrightInformation>Copyright © 2020 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ranjan</LastName><ForeName>Ruma</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>CSIR-National Botanical Research Institute, Lucknow, India; University of Lucknow, Lucknow, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Navin</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>CSIR-National Botanical Research Institute, Lucknow, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gautam</LastName><ForeName>Ambedkar</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>CSIR-National Botanical Research Institute, Lucknow, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar Dubey</LastName><ForeName>Arvind</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>CSIR-National Botanical Research Institute, Lucknow, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pandey</LastName><ForeName>Shyam Narayan</ForeName><Initials>SN</Initials><AffiliationInfo><Affiliation>University of Lucknow, Lucknow, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mallick</LastName><ForeName>Shekhar</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>CSIR-National Botanical Research Institute, Lucknow, India. Electronic address: shekharm@nbri.res.in.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Ecotoxicol Environ Saf</MedlineTA><NlmUniqueID>7805381</NlmUniqueID><ISSNLinking>0147-6513</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Arsenic</Keyword><Keyword MajorTopicYN="N">Chlorella sp.</Keyword><Keyword MajorTopicYN="N">Glutaredoxin</Keyword><Keyword MajorTopicYN="N">Glutathione-S-transferase</Keyword><Keyword MajorTopicYN="N">Oryza sativa L.</Keyword><Keyword MajorTopicYN="N">SAMT</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>04</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>09</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>09</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>12</Day><Hour>20</Hour><Minute>9</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33045435</ArticleId><ArticleId IdType="pii">S0147-6513(20)31255-0</ArticleId><ArticleId IdType="doi">10.1016/j.ecoenv.2020.111418</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Ranjan, Ruma" sort="Ranjan, Ruma" uniqKey="Ranjan R" first="Ruma" last="Ranjan">Ruma Ranjan</name></noRegion><name sortKey="Gautam, Ambedkar" sort="Gautam, Ambedkar" uniqKey="Gautam A" first="Ambedkar" last="Gautam">Ambedkar Gautam</name><name sortKey="Kumar Dubey, Arvind" sort="Kumar Dubey, Arvind" uniqKey="Kumar Dubey A" first="Arvind" last="Kumar Dubey">Arvind Kumar Dubey</name><name sortKey="Kumar, Navin" sort="Kumar, Navin" uniqKey="Kumar N" first="Navin" last="Kumar">Navin Kumar</name><name sortKey="Mallick, Shekhar" sort="Mallick, Shekhar" uniqKey="Mallick S" first="Shekhar" last="Mallick">Shekhar Mallick</name><name sortKey="Pandey, Shyam Narayan" sort="Pandey, Shyam Narayan" uniqKey="Pandey S" first="Shyam Narayan" last="Pandey">Shyam Narayan Pandey</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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